組織化学と顕微画像解析を利用した運動ニューロンの HRP 逆行性輸送量の測定法に関する 2, 3 の検討

概要

論文の詳細を見る
We have developed a method for measuring the amount of retrogradely transported HRP in the rat masseteric motor neuron using a modification of Mesulam's HRP histochemical protocol and an image processing system combined with a light microscope and a TV camera. Some factors affecting the reproducibility of the production and measurement of a colored reaction product after the HRP histochemical procedure were examined with the spectrophotometry in vitro and the image processing system for the tissue section. The results obtained were as follows : 1) In the TMB procedure for HRP activity, the difference in freshness of the solution A containing sodium pentacyanonitrosylferrate and carrageenan resulted in a conspicuous change of the amount of HRP reaction product. 2) When fine dark blue reaction products scattered in a 60 μm-thick section were measured with the light microscope of the image processing system, the difference in focusing affected little the measured values. 3) A little changing voltage of a light source of the microscope brought much difference in the measured values.
九州歯科学会の論文
1991-04-25