Molecular Cloning and Sequencing of Myosin Light Chains in Human Megakaryoblastic Leukemia Cells
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概要
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Myosin light chain genes of hematopoietic cells have yet to be characterized. We cloned the full-length cDNAs of 20 kDa regulatory myosin light chain (MLC-2) and 17 kDa essential myosin light chain (MLC-3) from Meg-01, a human megakaryoblastic leukemia cell line. Both MLC-2 and MLC-3 gene are transcribed ubiquitously in various hematopoietic cells. The MLC-2 open reading frame of 516 nucleotides encoding a protein of 172 residues was detected in cloned cDNA of 967 nucleotides. The Ca^<2+>-binding domain and five phosphorylation sites were highly conserved. The deduced amino acid sequence has a 99.4% and 100% homology with that of human fetus brain and human lymphocyte, respectively. The MLC-3 open reading frame of 453 nucleotides encoding a protein of 151 residues was detected in cloned cDNA of 742 nucleotide. The MLC-3 protein is 99.3% identical to that of human fibroblasts. These results suggest that hematopoietic myosin light chain proteins are similar to those of other nonmuscle cells and smooth muscle, thus differing from skeletal and cardiac muscles.
著者
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HIGASHIHARA Masaaki
Department of Hematology, Kitasato University School of Medicine
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Higashihara Masaaki
Fourth Department Of Internal Medicine Kitasato University School Of Medicine
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Higashihara Masaaki
あけぼの病院 内科
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Higashihara Masaaki
Section Of Hematology Fourth Department Of Internal Medicine Kitasato University School Of Medicine
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Higashihara Masaaki
北里大学 医学部第4内科(血液内科)
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Horie R
Department Of Hematology Kitasato University School Of Medicine
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Horie R
Fourth Department Of Internal Medicine Kitasato University School Of Medicine
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Horie Ryouichi
Department Of Internal Medicine School Of Medicine Kitasato University
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Higashihara Masaaki
Fourth Department Of Internal Medicine School Of Medicine Kitasato University
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Kohri Mika
Fourth Department Of Internal Medicine School Of Medicine Kitasato University
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Horie R
Univ. Tokyo Tokyo Jpn
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WATANABE Mariko
Fourth Department of Internal Medicine, School of Medicine, Kitasato University
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TAKAISHI Masaaki
Fourth Department of Internal Medicine, School of Medicine, Kitasato University
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HORIE Ryouich
Fourth Department of Internal Medicine, School of Medicine, Kitasato University
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Watanabe M
Dep. Of Hematology Internal Medicine Kitasato Univ. School Of Medicine
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Takaishi M
Kitasato Univ. School Of Medicine
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Takaishi Masaaki
Fourth Department Of Internal Medicine Kitasato University School Of Medicine
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