極性固定相を用いたイオン対高速液体クロマトグラフィーによるアルキル第4級アンモニウム塩の分離挙動

概要

論文の詳細を見る
The conditions for separation of alkyl quaternary ammonium salts were examined by ion pair high performance liquid chromatography using μ Bondapak CN as a polar stationary phase. Alkyl (C_<10>〜C_<16>) benzyldimethylammonium chlorides (benzalkonium chloride) and alkyl (C_<10>〜C_<16>) pyridinium chlorides were used as the quaternary ammonium salts. The retention behavior and the separability of the alkyl quaternary ammonium salts were evaluated in terms of solvent composition, the type of counter ions and the concentration of counter ions for forming ion-pair complex in mobile phase. The retention volumes of the alkyl quaternary ammonium salts increase with the increase in the water content of CH_3OH-H_2O and decrease with the increase in the length of alkyl chain of counter ions and the concentration of counter ions in the mobile phase. These alkyl quaternary ammonium salts were separated satisfactorily with a mobile phase of CH_3OH-H_2O (60/40, v/v) containing 1.0 × 10^<-2>M 1-pentane sulfonic acid (PIC-B-5 reagent). The relations between the logarithmic retention volumes (V-R) of the compounds and the alkyl chain length in alkylbenzyldimethylammonium chlorides and alkylpyridinium chlorides were shown to be Linear. The present method was applied to the separation of alkyl quaternary ammonium salts in imported samples, and satisfactory results were obtained.
社団法人日本分析化学会の論文
1984-06-05