極性固定相を用いたイオン対高速液体クロマトグラフィーによる芳香族スルホン酸塩の分離挙動

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The conditions for separation of aromatic sulfonates were examined by ion-pair high-performance liquid chromatography using μ Bondapak NH_2 as a polar stationary phase. Benzene monosulfonate, benzene 1,3-disulfonate, α-and β-naphthalene monosulfonates, three isomers of naphthalene disulfonates, and naphthalene 1,3,5-trisulfonate were used in this study. The influences on the elution behavior of aromatic sulfonates were evaluated in terms of solvent composition, the type of counter ion, the concentration of counter ion for forming ion-pair complex and pH value in mobile phase. Furthermore, the results obtained were compared with that of non-polar stationary phase such as octadecyl silanized silica gel. It is shown that the retention volume of aromatic sulfonates increases with increasing of the water content in H_2O-CH_3OH and the length of alkyl chain of counter ions and with lowering of pH value in the mobile phase. On the contrary, the retention volume decreases with increasing of counter ion concentration in the mobile phase. Except for the effect of the concentration of counter ions, above results were similar to those of reversed-phase ion-pair chromatography using non-polar stationary phase. These aromatic sulfonates were separated satisfactorily with a mobile phase of water-methanol (80/20 v/v) containing 2.5×10^<-3>M PIC-A reagent at pH 7, when μ Bondapak NH_2 was used as a stationary phase. It is found that this method is applicable to the separation of aromatic sulfonates in practical imported samples.
社団法人日本分析化学会の論文
1983-07-05

極性固定相を用いたイオン対高速液体クロマトグラフィーによる芳香族スルホン酸塩の分離挙動

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