ランタン-アリザリンコンプレクソン-フッ素三元錯体の薄層クロマトグラフィー挙動

概要

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The thin-layer chromatographic behavior of a lanthanum-alizarin complexone (ALC)-fluoride complex was investigated with particular interest being paid to the separation of the ternary complex from La-ALC. Although the spectral shift of the La-ALC chelate upon binding to the fluoride ion is widely used for the spectrophotometric determination of fluoride ion, there do not appear to be any reports on thin-layer chromatography (TLC) for the ternary complex (La-ALC-F). In this study, it was found that when developed with 60% ethanol on a C_18-bonded silica thin-layer plate, the ternary complex was easily eluted, forming a sharp band. Conversely, the La-ALC chelate was immobilized at the spotting position as a fleck. Furthermore, when a bluish-purple band was separated from a reddish-purple fleck, the ternary complex faded in a few minutes, whereas, when the band remained inside the fleck, no fading took place. These results are interpreted in terms of the dissociation of the labile complex caused by separation from the excess reagent. Consequently, the concentration of the La-ALC-F band across the center of the fleck provides a sensitive visualization of the fluoride ion down to 0.1 ppm.
社団法人日本分析化学会の論文
2002-09-05