鉄(II),1,10-フェナントロリン及びテトラヨードフルオレセインから成る三元錯体の溶媒抽出

概要

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The ternary complex of iron (II)-1, 10-phenanthroline (phen) with tetraiodofluorescein (TIF) can be extracted quantitatively into nitrobenzene from alkaline solution (pH 7.3〜8.3). The extracted complex has a composition of iron (II) : phen : TIF=1 : 3 : 2. This extraction system was applied to the spectrophotometric determination of iron. The recommended procedure is as follows. Take a sample solution containing up to 0.03 μmol of iron in a separatory funnel, add 0.5 cm^3 of 6.0 × 10^<-3> mol dm^<-3> L-ascorbic acid solution {to protect from aerial oxidation of iron (II) during extraction process}, 0.5 cm^3 of 3.0×10^<-3>mol dm^<-3> phen solution, 6 cm^3 of 0.2 mol dm^<-3> phosphate buffer solution (pH 8.0) and 0.5 cm^3 of 1.2×10^<-3>mol dm^<-3> TIF solution and dilute the mixture up to 30 cm^3 with water. After standing for 5 min, extract the ternary complex by shaking with 10 cm^3 of nitrobenzene for 5 min. Aitre centrifuging to separate the phases, measure the absorbance of the organic phase at 555.5 nm against the reagent blank, Beer's law is held up to 0.003〜0.03 μmol of iron and its molar absorptivity at 555.5 nm is 2.1×10^5 dm^3 mol^<-1> cm^<-1>. The extraction system for iron (II) becomes more selective when EDTA is added to a solution after the formation of metal-phen complexes. Nickel and perchlorate ions seriously interfere with the determination of iron.
社団法人日本分析化学会の論文
1986-02-05