ビタミン誘導体の微生物にたいする作用 : (I)S-Benzoylthiamine monophosphateの酵母にたいする作用

概要

論文の詳細を見る
The vitamin activity and the mode of action of S-benzoylthiamine monophosphate (BTMP) on yeast was described in this paper. This compound is reported to have a superior affinity to animal tissues than thiamine hydrochloride, perhaps because of its thiol structure with both lipophilic and hydrophilic groups. BTMP showed an obvious stimulating activity, though somewhat smaller than that of thiamine, on the growth and the fermentation of yeast, in contrast to its ineffectiveness on lactobacilli. To elucidate the difference between the effects on animal and yeast, the rate of exhibition of the activity, the rate of the absorption into yeast cell, and the forms of thiamine absorbed were compared with BTMP and thiamine. In the fermentation test for thiamine assay of Schultz et al., BTMP showed a longer induction period than thiamine, and the behavior was indentical with that of S-benzoylthiamine (SBT). As compared with the cell incubated with thiamine, the increase of total thiamine in BTMP-cell was slightly low, but the content of ester form was high. By paper chromatography the considerable part of the ester form was found to be thiamine monophosphate and acylated forms (BTMP and SBT) were not detected. From these results, it will be concluded that BTMP is absorbed into yeast cell as thiamine monophosphate after enzymatic cleavage of benzoyl group.
日本ビタミン学会の論文
1961-11-25