微生物における核酸系物質に対する酵素作用の研究 : (第1報)細菌のリボ核酸分解に対する界面活性剤の影響とヌクレオシドの生成

概要

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In oeder to produce nucleosides from RNA by some bacteria, the effect of surfactants on degradation of RNA was examined.1) Screening tests with bacteria having both the enzymes with degrading action on RNA (or reducing mononucleotide into nucleoside) and enzymes with the adenyl deaminase activity were carried out.As a result of these tests, the intact cells obtained from several strains of genus Bacillus and Pseudomonas showed high productivity of nucleosides from RNA and mononucleotide with an addition of a surfactant.2) It was found that effective surfactants for the nucleoside formation inhibit the nucleoside hydrolase activity and promote the RNA degradation.However, the intact cells obtained from Bacillus subtilis (an adenine requiring mutant) showed little hydrolytic ability for purine nucleoside and produced inosine and guanosine from RNA.3) The optimum pH value for the nucleoside formation from RNA was in the range of 5-7.The maximum concentration of RNA for the nucleosides production by a cultural broth under the optimum conditins was less than 3.5g per 100ml of broth in B. cereus, and less than 6-8g per 100ml of broth in B. subtilis mutant and Pseudomonas riboflavinus.
社団法人日本生物工学会の論文
1968-01-25