ジヒドロストレプトマイシンによるPseudomonas aeruginosaの栄養要求変異株の分離について

概要

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The possibility of isolating auxotrophic mutants from much larger populations of one penicillin-insensitive strain of Pseudomonas aeruginosa, strain No.2120,was inferred from the results of the experiments in which under suitable conditions dihydrostreptomycin was more bactericidal for growing cells than for resting cells of this strain. Cells of Ps.aeruginosa No.2120 was irradiated with ultraviolet by a Matsuda 15W germicidal lamp at a distance of 40 cm for 10 seconds to reduce the viable count from 1×10^7 viable cells per ml to 1×10^4 viable cells per ml, stored in the dark for 2 hours, then culltivated in nutrient broth at 30℃ for 6 hours. After washing cells, the cells were preincubated in Cray and Tatum's minimum medium at 30℃ for 2 hours, then exposed to dihydrostreptmycin, 400μg per ml, in minimum medium at 30℃ for 7 hours. Auxotrophic mutants were enriched about one hundred times at best by this antibiotics treatment. There was no evidence that dihydrostreptomycin was mutagenic for this strain, and not all the mutants isolated by this technique were resistant to dihydrostreptomycin.
社団法人日本生物工学会の論文
1966-05-25