微生物によるL-アスパラギン酸よりL-アラニンの調製 : (第1報) 菌株の選択ならびに培養条件の検討

概要

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A screening test on strains possesing L-aspartic acid decarboxylase was performed. Among many isolates brom natural origin and strains in type culture collection tested, bacteria which belong to Xanthomonas or Pseudomonas species were found to have strong activity. The isolate No. 531,identified to be a strain of Xanthomonas oryzae, had an extremely strong decarboxylase activity. Using this strain, the conditions for the cell growth and enzyme production were studied. In general, the addition of organic nitrogen such as casamino acid favored cell growth and enzyme production, and L-alanine, L-glutamic acid and L-aspartic acid addition were especially effective. As an inorganic nitrogen source, NH_4Cl and NH_4-tartarate were most suitable. Furthermore, the results obtained from cultivating this microorganisms in 25 l. jar fermentors showed that maintaing a medium pH within an adequate range (pH7-8) was very effectve for the cell growth and enzyme production. L-Aspartic acid was quantitatively decarboxylated to L-alanine by this enzyme preparation. The reaction product was isolated by ion exchange resin, eluted with ammonia solutin, crystallized and proved to be an L-form of alanine.
社団法人日本生物工学会の論文
1965-02-25