フェニルプロパノールおよび関連化合物による清酒酵母の呼吸欠損変異株の誘発

概要

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Induction of a respiration-deficient mutant of Saccharomyces sake strain Kyokai no. 7 by four kinds of phenyl propanols and six related compounds was studied. Benzyl alcohol showed higher minimum inhibitory concentration (MIC) than phenol, and the more higher the carbon number of side chain, the lower the MIC was. Induction of petite colonie mutants was detected by overlaying triphenyltetrazolium chloride on the agar plate. When a compound was added directly to the medium, frequency of petite colonies was higher than when an ethanolic solution of the same compound was added. Maximum induction frequencies, 79.7% and 20.9%, were obtained by direct addition and as alcoholic solution of α-phenethyl alcohol, respectively. The other compounds also showed induction effects to some degree.Petite colonie mutants thus obtained were confirmed to be respiration-deficient mutants as they showd no growth on sodium lactate agar, no oxygen uptake, and did not have cytochrome a and b.
公益社団法人日本生物工学会の論文
1976-01-25