Effects of Methanol and Magnesium Chloride on the Induction of Respiration-Deficient Mutants in Yeast by Metal Ions

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The effects of methanol and magnesium chloride on the induction of cytoplasmic respirationdeficient (RD or petite) mutants of yeast, Saccharomyces cerevisiae, by metal compounds were investigated. ZnCl_2,HgCl_2,NiCl_2,CdCl_2 and MnCl_2 induced 1.8-2.2% RD mutants at concentrations where almost no growth inhibition was observed. Induction of RD mutants by these metal compounds decreased to the spontaneous level (1.4%) in 4% methanol. The effect of methanol was weaker on RD mutant induction by PbCl_2 and CuCl_2. The induction of RD mutants by CoCl_2 was not affected by methanol. NiCl_2 and CdCl_2 were strongly mutagenic at higher concentrations, and the mutagenicity of these metal compounds was also repressed by 4% methanol. The frequency of RD mutants induced in the presence of 1×10^<-2>M MnCl_2 was increased from 13.5 to 60.7% by the addition of methanol. This increase of the induction of RD mutants in the presence of MnCl_2 and methanol was antagonized almost completely by the addition of MgCl_2 to the culture medium. The same effect of MgCl_2 was observed in the absence of methanol. The effects of methanol and MgCl_2 on the induction of RD mutants in yeast are discussed.
公益社団法人日本薬学会の論文
1985-04-25