Rhizopus属と細菌の関連作用によるフマル酸醗酵からコハク酸への転換醗酵 : (第4報)Rhizopus arrhizusとAerobacter aerogenesの組合わせによるコハハク珀酸の生産および転換醗酵機作

概要

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After Rhizopus arrhizus NRRL 1526 was grown in a shaking culture in medium containing 8% glucose at 32℃ for 2 days, Aerobacter aerogenes AHU 1341 was inoculated into the broth, then grown in a mixed, stationary cluture for more 2 days. As a result, fumaric acid fermentaiton by R. arrhizus was converted to succinic acid fermentation by the aciton of A aerogenes and a succinic acid yield as high as 51.75% (based on the initial glucose) was obtained. In this fermentation, it was found thant the conversion of fumaric acid to succinic acid by the mixed culture proceeded much more efficiency than that by a single culture of A. aerogenes. Succinic and acetic acids were formed at a molar ratio of 2 : 1. Succinic acid formation depended upon the erduction of fumaric acid by the fumarate erductase of A. aerogenes, requiring NADH_2 which was released during the metabolism of fumaric acid to acetic acid. Addition of a small amount of NADH_2 to the medium containing fumaric acid as the sole carbon source raised the succinic acid produciton by A. aerogenes to the conversion level by the mixed culture of R. arrhizus and A. aerogenes. Therefore, it seemed thant the enhancemenct of succinic acid produciton from fumaric acid by the above mixed culture was caused by the activation of fumarate reductase by NADH_2 which was probably produced in course of glucose metabolism by R. arrhizus.
社団法人日本生物工学会の論文