Rhizopus属と細菌の連関作用によるフマル酸醗酵からアスパラギン酸への転換醗酵 : (第2報)Rhizopus属とProteus vulgraisの組合わせによるアスパラギン酸の生産

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Conversion of fumaric acid fermentation to aspartic acid fermentation was performed by the association of a fumaric acid-producing Rhizopus and Proteus vulgaris having a high aspartase activity. After R. chinensis AHU 6508 was shake-cultured in a medium containing 10% of glucose at 32℃ for 5 days, 2.0×10^9 cells of P. vnlgaris AHU 1144 was inoculated into the broth and 2.3g of (NH_4)_2HPO_4 was added at the same time. The broth was then incubated for another day under the same conditions. A 26.4% yield of aspartic acid (based on the initial glucose) was obtained.In this fermentation it was found that the aspartic acid yield depended upon the quanity of fumaric acid produced by Rhizopus.In order to obtain a higher yield of aspartic acid, R. arrhizus NRRL 1526,which produced fumaric acid in yields over 60%, was used. In a medium containing 4 to 6% of fumaric acid and 4.6% of (NH_4)_2HPO_4 whose composition, expect for the carbon and nitrogen sources, was the same as the medium for fumaric acid fermentation by R.arrhizus, P. vulgaris converted about 90% of fumaric acid to aspartic acid within 24 hr. R. arrhizus did not have any aspartase activity. When P. vulgaris was grown in a mixed culture with R.arrhizus, conversion of fummaric acid to aspartic acid was a little depressed and small amounts of malic acid were accumulated as a by-product. Howere, when R.arrhizus was shake-cultured to produce fumaric acid at 32℃ for 3 days in the medium containing 8% of glucose, and then 2.3g of (NH_4)_2HPO_4 were added to the broth and associated with P. vnlgaris for one day under the same conditions, aspartic acid yields as high as 62.4% (based on initial glucose) were secured.
社団法人日本生物工学会の論文

Rhizopus属と細菌の連関作用によるフマル酸醗酵からアスパラギン酸への転換醗酵 : (第2報)Rhizopus属とProteus vulgraisの組合わせによるアスパラギン酸の生産

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