Rhizopus属と細菌の連関作用によるフマル酸醗酵からコハク酸への転換醗酵 : (第3報)Aerobacter aerogenesによるフマル酸からコハク酸への転換

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Fundamental experimetns were carried out to establish the conversiton of fumaric acid fermentation to succinic acid fermentation by the association of fumaric acid-producing Rhizopus and Aerobater aerogenes having a high ability to convert fumaric acid to succinic acid without the addition of a hydrogen source, such as glucose, required for the reduction of fumarate. Convension of fumaric acid by A.aerogenes AHU 1341 proceeded much more rapidly in stationary culture than in shaking cluture. Ca-fumarate was quite desirable for the conversion in compariso with K_-, NH_<4->, or Na-fumarate. The initial pH range between 6.5 and 8.5 was optimum. Favorable inoculum size was 1.6×10<10> cells per ml of medium. Succinic acid yields from malic acid were as high as those from fumaric acid. When A. aerogenes was grown in a stationary culture at 32℃ in a medium containing 4% of fumaric acid under favorable conditions, the succinic acid yield after 24 hours was more than 40%; the conversion was completed with a succinic acid yield as high as about 56% after 48 hours. At the same time, acetic acid was also accumulated with a yield of 44%.Rhizopus arrhizus NRRL 1526 accelerated the conversion by A. aerogenes; a more than 60% final yield of succinic acid was obtained.
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