菌類の蛋白分解酵素に関する研究 : (第1報)金属キレート化合物のAspergillus oryzaeのアルカリ性プロテアーゼ作用に及ぼす影響

概要

論文の詳細を見る
Considering a study on the effects of metal chelate compounds on proteases would be useful for examining the complex enzyme system, the authors investigated catalytic effects of copper (II) chelates of 1,10-phenanthroline (Phen) and 2,2'-dipyridyl (Dipy) on a alkaline protease produced by Aspergillus oryzae (abbr. APase), a species of serine enzymes. Out of many chelate-forming compounds tested, only Phen and Dipy chelates with cupric ion showed negative effects on the caseinolytic activity of APase.It was concluded that Phen-Cu compounds inhibit APase only on condition that they are diaquo and monohydroxo unsaturated (1 : 1) chelates. This conclusion is based on the following facts : (1) An increase in pH of the reaction mixture resulted in a decrease in degree of the inhibition of APase by Phen-Cu chelate and, when the pH was higher than 10,probably an inactive olated dimer was formed.(2) In case of an excessive presence of the Phen concentration in proportion to cupric ion in the reaction mixture, equilibrium of Phen-Cu chelate shifted to saturated (2 : 1) one, which no longer displayed any negative action on the APase activity.Phen-Cu chelate protected APase from its inactivation by a heat treatment.
社団法人日本生物工学会の論文
1969-01-25