グルタミン酸醗酵に関する研究 (第12報) : 醗酵液中グルタミン酸の分離 (その2)

概要

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Following the previous papers, this is a report of studies on the recovery of glutamic acid from culture broth by means of electrophoretic concentration using ion exchange membranes. The electrolytic cell was subdivided into a number of compartments with alternately aligned cation and anion exchange membranes. When the culture broth was subjected to electrolysis in the above cell, ions were transfered out of every other compartment (D) and concentrated in neighboring chambers (C). In one series of experiments, about 90% of glutamic acid in D was transfered to C in 150〜360 min depending on current density which ranged from 1.4 to 0.7amp./dm^2. As the electrophoresis proceeded, the concentration of electrolytes in D constantly decreased, causing the decline of current density and preventing the efficient operation. When the broth was continuously charged into D and the dilluted solution discharged from the same compartments, the decline of current density was prevented, enabling the efficient operation.It was quite necessary to clarify and purify the broth before carrying out the electrophoretic concentration.
社団法人日本生物工学会の論文
1959-08-15