グルタミン酸醗酵に関する研究 (第2報) : 培地組成(その1) 炭素源について

概要

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Among the carbohydrates tested for the glutamic acid formation by Strain No. 1942,glucose and sucrose gave the highest yield. While a hydrolysate of cassava starch proved to be useful in place of glucose, cane sugar did not always give the reproducible results owing to the different content of some factors in sugar, such as biotin. A high concentration of biotin in a medium will only promote the propagation of the micro-organism without any accumulation of glutamic acid. Tests on black strap molasses were discouraging.Since cassava starch is one of the possible raw materials for the fermentative production of glutamic acid, an investigation was carried out to determine the optimum concentration of glucose. With 0.8% of urea in the medium, 5% of glucose gave the highest yield on the basis of the glucose consumed. It is also recognized that the concentration of glucose should be carefully adjusted according to the concentration of urea. Thus 3,5,7 and 10% of glucose require 0.5,0.8,1.1 and 1.5% of urea respectively. Considering that a high final concentration of glutamic acid in a broth is industrially important and that the yield of glutamic acid based on the initial glucose present in a medium is significant, rather than one based on the glucose consumed, more than 7% of initial concentration of glucose is desirable. Too high concentration of glucose does not necessarily give favorable results.
社団法人日本生物工学会の論文
1959-08-15