グルタミン酸醗酵に関する研究 (第11報) : 醗酵液中グルタミン酸の分離 (その1)

概要

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Two methods of recovering glutamic acid from the culture broth were evaluated; (1)precipitation of glutamic acid from concentrated culture broth by adjusting pH to 3.2,and (2) separation of glutamic acid in a form of hydrochloride. By the first method cells and other insoluble materials were removed by filtering the broth. The filtrate was concentrated and the pH adjusted to 3.2. The glutamic acid thus obtained was too impure to be purified simply by washing, but highly increased its purity when dissolved in hydrochloric acid and reprecipitated with caustic soda. The yield was a little low and yet could not be improved by increasing the degree of concentration. By the second method the culture broth was concentrated and hydrolyzed with hydrochloric acid. The hydrolysate was filtered, concentrated, and the glutamic acid hydrochloride separated and converted to glutamic acid of high purity. The culture broth which had not been filtered gave a higher yield than that filtered.
社団法人日本生物工学会の論文
1959-08-15