グルタミン酸醗酵に関する研究 (第3報) : 培地組成 (その2) 窒素源について

概要

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Among the nitrogen compounds tested for the glutamic acid formation by Strain No. 1942,urea, (NH_4)_2SO_4 and NH_4Cl gave the highest yield, and their optimum concentrations were 1.5%, 3.0%, and 3.0% respectively. While a sufficient supply of NH_4 is essential for the formation of glutamic acid, there are tendencies that urea increases and (NH_4)SO_4 (or NH_4Cl) decreases the pH of medium too seriously. In order to prevent these tendencies, two ways were devised, one being the incorporation of urea and (NH_4)_2SO_4 (or NH_4Cl), and the other being the gradual feeding of urea during the course of fermentation. Both proved to be effective, and the optimum proportion of urea and (NH_4)_2SO_4 was 1.2 : 2.0% or 1.5% : 1.5%, while the best method of gradual feeding of urea was the addition of 0.8% urea to the medium during the fermentation, the initial concentration of urea being 0.8%. The feeding of urea has a function of not only supplying NH_3 but also controlling the pH of medium. The investigations earried out along this line will be reported in a future paper.
社団法人日本生物工学会の論文
1959-08-15