ピリミジン部2位置換ビタミンB_1誘導体の補酵素作用 : (III)2価金属イオンによるアポトランスケトレースの活性化

概要

論文の詳細を見る
The activation of brewer's yeast apotransketolase with divalent cations was observed effectively in a low thiamine pyrophosphate concentration. Relative activities were observed in the order of Ca^<2+>>Mn^<2+>>Mg^<2+>>Cd^<2+> and no activity with Zn^<2+>, Cu^<2+>. Holotransketolase which was reconstituted with Ca^<2+> or Mn^<2+> showed negligible resolution in gelfiltration (Sephadex G-25), but that reconstituted with Mg^<2+> or Cd^<2+> lost their original activities in gelfiltration and reactivated by the incubation with thiamine pyrophosphate and Mg^<2+> as high as 38%, 51% respectively. The inhibition of transketolase with Zn^<2+> was removed by means of gelfiltration, while that with Cu^<2+> was not removable.
日本ビタミン学会の論文
1971-12-25