脚氣患者の糞便から分離せる一細菌のアノイリナーゼの性状に就て

概要

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I treated the solution of beriberi patient's faeces (diluted as ten times) for 20 min. at 60℃, and then spreaded one platin-loop of it on the bouillon agar plate.The bacterium which secretes aneurinase was isolated and its properties were observed.The isolated bacterium is a species belonging to the genus Bacillus, and it seems to be a mutant obtained by the heat treatment.In the bouillon medium, aneurinase secreted by this bacillus decreases after culture for 3 3 days at 37℃.The optimum activity of aneurinase lies at pH 4〜5 and 40℃.Aneurinase loses its activity by dialysis, but restores it by adding boiled enzyme solution into the indiffusable liquid left behind. Thus it will be concluded that the bacillus aneurinase contains a heat stable coenzyme.
公益社団法人日本生物工学会の論文
1951-11-15