Flavobacterium sp.によるビタミンB_6の生合成

概要

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Flavobacterium sp. 238-7 excreted a considerably high amount of vitamin B_6 i.e. 20 μg/ml, in the form of pyridoxine into the culture medium. The cellular productivity was higher than that of other microorganisms. A resting cell system of vitamin B_6 biosynthesis was developed. In screening of compounds effective for improvement of the vitamin production, some nucleosides and nucleotides were found to have a strong stimulating effect on the biosynthesis. Some amino acids were also effective. Though the addition of glycolaldehyde did not increase the amount of vitamin B_6 in the system, a significant enzyme activity for reduction of glycolate to glycolaldehyde was found in a membrane fraction of the cell free extract. The enzyme catalyzed the reduction but not the oxidation, namely the reverse reaction, and was therefore designated as glycolate reductase. A positive correlation was observed between the enzyme activity and the biosynthesis of vitamin B_6 during the cultivation. The high vitamin productivity of the bacterium could be explained by lack of regulation of vitamin B_6 biosynthesis in the growing and resting cell systems and by that of glycolate reductase.
日本ビタミン学会の論文
1984-10-25