ソルボリシスパルプ化廃液でのAureobasidium pullulans菌体生産の培養条件

概要

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The optimum cultural conditions were investigated for the cell mass production of strain A. pullulans IFO 6353 which has found to be the most promisting organism that uses xylan in the solvolysis pulping waste liquor.The optimum cultural conditions were 29℃, initial pH 60.-6.5. Ammonium sulfate and L-asparagine were suitable as supplementary nitrogen sources. The optimum concentrations of supplemental nutrients were 0.5% ammonium surfate, 0.5-1.0% potassium dihydrogenphosphate, and 0.1% magnesium sulfate. Further, the addition of yeast extract greatly increased the cell mass production. The cell mass reached 7.5g per l of medium after 3 days under the optimum conditions(yeast extract, 0.25%). After 3 days of cultivation, A. pullulans IFO 6353 had used about 70% of the mono-, oligo-, and poly-saccharides in the waste liquor as one of the carbon sources.
社団法人日本生物工学会の論文
1986-11-25