螢光抗体法による植物ウイルス増殖の追跡 : 1. タバコ新葉中における TMV の増殖

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Localization and translocation of tobacco mosaic virus in the cells and tissues of the systemically infected young leaves of Nicotiana tabacum L. var. Xanthi was investigated by means of the fluorescent antibody technique. The specific staining resulting from the serological reaction between the virus antigen and its specific antiserum was found at first in both the palisade and spongy parenchyma tissues surrounding the peripheral portions of vascular bundles, and subsequently the antigen was detectable soon in the neighboring regions. The first change to be discerned in the infected cell as a result of the virus multiplication was the formation of specifically stained small granules in cytoplasm, follolwed by their gradual enlargement, scattering by the protoplasmic steaming into reticulate arrangement of the antigen. At a later stage of virus multiplication, the viral antigen seemed to be concentrated around the unstained nucleus, where the specific staining was markedly intensified. The granules irregularly distributed in the cell seemed to lie in contact with chloroplasts but not near the nucleus. The tissue sections prepared from leaves showing typical mosaic symptoms were found by the staining technique to be composed of two distinct areas, clearly stained and unstained. Virus antigen was demonstrated in hair cells, epidermal cells, guard cells of the stomata, calcium-oxalate cells orf the spongy parenchyama and aosl in the phloem.
日本植物病理学会の論文
1965-05-31

螢光抗体法による植物ウイルス増殖の追跡 : 1. タバコ新葉中における TMV の増殖

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