オオムギ斑葉モザイク病罹病植物の寒天ゲル中沈降反応による抗原分析

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1. This paper deals with the resuts of cross-absorption tests of antisera for barley stripe mosaic virus, purified by ultra-centrifugation or by salting-out with ammonium sulfate, from juices of diseased barley, wheat, and corn plants. Use was made of the agar-gel diffusion method. When the antisera had been absorbed by homologous healthy plant juices, there always appeared a single precipitation band against diseased plant juices, either homologous or heterologous. 2. This precipitation band was usually located near the outer hollows filled with the antigens, and curved towards both ends to show a sickle or bow shape. As this band disappeared when the antisera had been absorbed by diseased plant juice or by purified virus suspension, this precipitation pattern was considered to represent a specific reaction between the virus-antigen and its corresponding antibody. 3. All other precipitation bands were formed usually midway between two hollows filled with the antigen and the antiserum. Because the antigenic components in these reactions diffused more rapidly jn the agar layer than did the virus-antigen, these components were considered to be relatively small particles, and seemed to be normal plant proteins. 4. It was recognized that antisera prepared with barley plant juices purified by salting-outwith, ammonium sulfate contained at least four distinguishable antibcdies, while antisera prepared with wheat, and corn plant juices contained five and four distinguishable antibodies, respectively, all of which were considered to be antibcdies against normal plant proteins. 5. In the absorption tests, no antigen-antibody system common to normal plant proteins of the three different host plants, except the virus-antigen and its corresponding antibody, have been found by the use of agar-gel diffusion tests. 6. The precipitation band resulting from the reaction between the virus and its corresponding antiserum was considered to occur on the locus of two intersections of two circles representing the diffusion fronts of both antigen and antibody, and mathematical equation for the locus was obtained.
日本植物病理学会の論文
1963-01-30

オオムギ斑葉モザイク病罹病植物の寒天ゲル中沈降反応による抗原分析

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