螢光標識抗体によるスライド凝集反応について

概要

論文の詳細を見る
Using the fluorescein-1abeled antibodies, s1ide agglutination tests were emp1oyed to detect several plant viruses under dark field i11umination in a Nikon microscope connected with a mercury vapour lamp(Chiyoda AHL 250). Positive reactions were recognized with tobacco masaic virus, potato virus X and Y, and rice dwarf virus by their corresponding conjugated antisera. A drop of crude sap from infected plants was mixed with about one third to one fourth its vo1ume of the concentrated fluorescein-1abeled antibody solution on a microscope slide, stirred with the corner of a cover glass and covered with same. This method is available for detecting the antigen-antibody complexes stained specifica11y in the mixtures despite the weak reaction. This method is very exce11ent because it is easy to apply, and the quantity of both antiserum and antigen to be required is very 1ittle, and specificity of the reaction can be directly confirmed under a microscope. A high sensibi1ity of the slide agglutination test was ascertained by experimental results with box titration using each virus and its homo-1ogous conjugated antiserum.(Received September 14, 1965)
日本植物病理学会の論文
1966-06-30