A method of cell-sheet preparation using collagenase digestion of salmon atelocollagen fibrillar gel.
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概要
- 論文の詳細を見る
We prepared a cell sheet by using collagenase treatment to digest salmon atelocollagen fibrillar gel (SAC gel) on which human periodontal ligament (HPDL) cells had been cultured. The SAC gel was found to be digested completely within 2 h at a concentration of 50 U of collagenase per mg of collagen. The SAC gel on which HPDL cells were cultured for 10 d was treated with collagenase, resulting in the formation of a detached and shrunken cell sheet. Immunostaining results showed that the cytoskeleton and fibronectin matrix level of the cell sheet were maintained after collagenase treatment. In addition, collagenase treatment had almost no effect on the activities of HPDL cells.
- 社団法人日本生物工学会の論文
- 2004-12-25
著者
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Satoh Yasuharu
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Tajima Kenji
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Munekata Masanobu
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Tajima K
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Satoh Y
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Munekata Masanobu
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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NAGAI NOBUHIRO
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University
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Yunoki Shunji
Division Of Frontier Research Creative Research Initiative "sousei" (cris) Hokkaido Univer
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Nagai Nobuhiro
Division Of Frontier Research Creative Research Initiative "sousei" (cris) Hokkaido Univer
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Tajima Kenji
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Nagai Nobuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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YUNOKI SHUNJI
Ihara & Company Ltd
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Munekata Masanobu
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido University
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Tajima Kenji
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido University
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