Application of Elastic Salmon Collagen Gel to Uniaxial Stretching Culture of Human Umbilical Vein Endothelial Cells(CELL AND TISSUE ENGINEERING)
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概要
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In this study, we investigated the potential of an elastic salmon collagen gel(e-gel) for use as stretching culture scaffold. First, human umbilical vein endothelial cells(HUVECs) were cultured on the e-gel under static condition, and their growth was evaluated by DNA content measurement, MTT test, and scanning electron microscopy. The results demonstrated steady increases in cell number with culture time. Next, HUVECs were cultured on the e-gel under static condition for 2 d, then uniaxially stretched at a constant frequency(10% elongation at 1 Hz). After the stretching culture for 2 h, the cells oriented perpendicularly to the stretch direction. Moreover, the interleukin-6 and interleukin-8 productions of the cells significantly increased under the stretch condition compared with those under the static condition. These results were in good agreement with the published data in which an elastic silicone membrane was used as a scaffold. In conclusion, the e-gel can be used for stretching culture for vascular tissue engineering.
- 社団法人日本生物工学会の論文
- 2008-05-25
著者
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Munekata Masanobu
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Munekata Masanobu
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Nagai Nobuhiro
Creative Research Initiative "Sousei", Hokkaido University
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Nagai Nobuhiro
Creative Research Initiative "sousei"(cris) Hokkaido University
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Kanayama Toshiyuki
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido Uni
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Mori Kazuo
Ihara & Company Ltd.
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Kanayama Toshiyuki
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Mori Kazuo
Ihara & Company Ltd.
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