Effects of Endogenous Endo-β-1,4-Glucanase on Cellulose Biosynthesis in Acetobacter xylinum ATCC23769
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概要
- 論文の詳細を見る
Endo-β-1,4-glucanase (CMCax; EC 3.2.1.4) from Acetobacter xylinum ATCC23769 was expressed as a 6×His-tagged fusion protein in Escherichia coli. The optimal temperature, pH, K_m and V_<max> of the purified H is-tagged CMCax toward carboxymethyl cellulose were 50℃, 4.5, 20 mg/ml and 37.2 μM/min, respectively. The number of recognition residues of cello-oligosaccharide by this enzyme were five (cellopentaose) or longer, and the stereochemical course of hydrolysis was of the inverting type. Addition of a small amount (1.5 mg/l) of His-tagged CMCax into a culture medium enhanced cellulose production 1.2-fold. CMCax overproduction in A. xylinum also enhanced the yield of cellulose production. Transmission electron microscopic analysis revealed that the cellulose ribbons secreted from the CMCax overproducing strain were dispersed compared with those from the wild type strain in the same manner as by carboxymethyl cellulose addition. These results could suggest that CMCax from A. xylinum influences in cellulose ribbon assembly, which is considered to be a rate-determined process in cellulose synthesis.
- 社団法人日本生物工学会の論文
- 2002-09-25
著者
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Tajima Kenji
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Munekata Masanobu
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Takai M
Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University
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Takai Mitsuo
Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University
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Erata T
Univ. Tsukuba Ibaraki
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Tajima K
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Kono Hiroyuki
Division Of Biotechnology And Molecular Chemistry Graduate School Of Engineering Hokkaido University
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Munekata Masanobu
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Tajima Kenji
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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ERATA Tomoki
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University
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KAWANO SHIN
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University
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Munekata Masanobu
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido University
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Tajima Kenji
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido University
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