Isolation and Characterization of Bacillus sp. INT005 Accumulating Polyhydroxyalkanoate (PHA) from Gas Field Soil(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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A gram-positive bacterium (designated strain INT005) that accumulated polyhydroxyalkanoate (PHA) was isolated from gas field soil. From its morphological and physiological properties and the partial nucleotide sequence (about 500 bp) of its 16S rDNA, it was suggested that strain INT005 was similar to several species of the genus Bacillus. We confirmed that strain INT005 is a Bacillus sp. The PHA productivities of strain INT005 were higher than those of Bacillus megaterium and Ralstonia eutropha at 37-45℃ reported to date, and it was suggested that the PHA synthase of INT005 may exhibit moderate thermostability. The bacterium had the ability to produce poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), poly(3-hydroxybutyrate-co-4-hydroxybutyrate-co-3-hydroxyhexanoate), and poly(3-hydroxybutyrate-co-6-hydroxyhexanoate-co-3-hydroxyhexanoate) from the appropriate carbon sources. The PHA synthase from INT005 showed similar substrate specificity to those of class I and III PHA synthases and strain INT005 produced PHAs with various monomer compositions. From the analysis of monomer composition and PHA accumulation in the presence of acrylic acid, it was suggested that de novo fatty acid synthesis and β-oxidation are involved in the PHA synthesis of Bacillus sp. INT005. Since Bacillus sp. INT005 could synthesize PHA even at 45℃ and PHAs with various monomer compositions, and only one report on the cloning of the synthesis-related genes from a Bacillus species (B. megaterium) has been published; Bacillus sp. INT005 is thought to be very valuable source of PHA synthesis-related genes.
- 社団法人日本生物工学会の論文
- 2003-01-25
著者
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Satoh Yasuharu
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Tajima Kenji
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Munekata Masanobu
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering. Hokkaido Uni
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Tajima K
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Nakamura M
Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University
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Nakamura Maiko
Division Of Nuclear Medicine Pet Center And Department Of Radiology Kurume University School Of Medi
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Satoh Y
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Nakamura Masaaki
Department Of Chemical Engineering Nagoya University
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Munekata Masanobu
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Nishimura Daisuke
Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University
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Igari Takaaki
Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University
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Tajima Kenji
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido Unive
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Nakamura Maiko
Division Of Molecular Chemistry Graduate School Of Engineering Hokkaido University
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Munekata Masanobu
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido University
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Tajima Kenji
Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido University
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