Preparation and Characterization of Multilayered Hydroxyapatite/Silk Fibroin Film(CELL AND TISSUE ENGINEERING)

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概要

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• We prepared multilayered films consisting of silk fibroin (SF) and hydroxyapatite (HAp) by alternating lamination using untreated SF and HAp-deposited SF films. Untreated SF films were prepared from a regenerated SF solution by air drying. HAp-deposited SF films were prepared by soaking methanol-treated SF films containing >5 wt% CaCl_2 in a simulated body fluid with the ion concentration 1.5-fold higher than that of the standard one. The multilayered HAp/SF films had HAp layers with approximate thicknesses of 3-5 μm and SF layers with thicknesses of 40-70 μm. The bonding strength between the SF and HAp layers was significantly affected by temperature and compression time under the lamination method. The optimal conditions for achieving the maximum T-peel strength and β-sheet contents were determined to be 130℃ for 4 min. The Young's modulus of the multilayered films (133.4 MPa) was higher than that of the films consisting of SF alone (92.5 MPa) under swollen conditions. The biocompatibility of the HAp-deposited SF films was analyzed by culturing of osteoblasts (MC3T3-E1) on a film. The results indicate that HAp-deposited SF films and SF films show similar degrees of cell adhesion and alkaline phosphatase activities.

• 社団法人日本生物工学会の論文
• 2007-06-25

著者

• ASAKURA Tetsuo

  Department of Biotechnology, Faculty of Technology, University of Agriculture and Technology

• Asakura Tetsuo

  Department Of Biotechnology Tokyo University Of Agriculture And Technology

• YUNOKI SHUNJI

  Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University

• NAGAI NOBUHIRO

  Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University

• Yunoki Shunji

  Division Of Frontier Research Creative Research Initiative "sousei" (cris) Hokkaido Univer

• Nagai Nobuhiro

  Division Of Frontier Research Creative Research Initiative "sousei" (cris) Hokkaido Univer

• Nagai Nobuo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kino Rikako

  Faculty and Graduate School of Engineering, Hokkaido University

• Ikoma Toshiyuki

  Biomaterials Center, National Institute for Materials Science

• Tanaka Junzo

  Division of Frontier Research, Creative Research Initiative "SOUSEI" (CRIS), Hokkaido University

• Munekata Masanobu

  Faculty and Graduate School of Engineering, Hokkaido University

• Ikoma Toshiyuki

  Biomaterials Center National Institute For Materials Science

• Kino Rikako

  Faculty And Graduate School Of Engineering Hokkaido University:division Of Frontier Research Creativ

• Tanaka Junzo

  Division Of Frontier Research Creative Research Initiative "sousei" (cris) Hokkaido Univer

• Asakura Tetsuo

  Department Of Biochemistry And Life Sciences Faculty Of Technology Tokyo University Of Agriculture A

• Tanaka Junzo

  Division Of Frontier Research Creative Research Initiative "sousei" (cris) Hokkaido Univer

• Kino Rikako

  Faculty And Graduate School Of Engineering Hokkaido University

• Munekata Masanobu

  Division Of Biotechnology And Macromolecular Chemistry Graduate School Of Engineering Hokkaido University

• Yunoki Shunji

  Division of Frontier Research, Creative Research Initiative "SOUSEI" (CRIS), Hokkaido University

• Tanaka Junzo

  Division of Frontier Research, Creative Research Initiative "SOUSEI" (CRIS), Hokkaido University

• Kino Rikako

  Faculty and Graduate School of Engineering, Hokkaido University:Division of Frontier Research, Creative Research Initiative "SOUSEI" (CRIS), Hokkaido University

• ASAKURA Tetsuo

  Department of Biotechnology, Tokyo University of Agriculture and Technology

• Nagai Nobuhiro

  Division of Frontier Research, Creative Research Initiative "SOUSEI" (CRIS), Hokkaido University

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