Polymerization and Calcium Binding of the Tubulin-colchicine Complex in the GDP State(Biochemistry & Molecular Biology)

スポンサーリンク

概要

• 論文の詳細を見る

• The tubulin-colchicine complex instead of tubulin was used in an imidazole buffer throughout experiments. The interaction with calcium was examined, especially in the GDP state. The high affinity sites of calcium took part in the polymerization of the complex in the GTP state, while the low ones participated in the depolymerization. The complex had 2 high affinity sites with the dissociation constant of 11.5 × 10^<-6> M, and 16 low affinity sites with the dissociation constant of 2.27 × 10^<-4> M in the GTP state. In the case of GDP state, the dissociation constant of the high affinity site was 7.2 × 10^<-6> M, and the low affinity site was not observed. The ultracentrifugal experiment indicated a little compact structure in the GTP state compared with the GDP state. This agreed with the results of calcium binding.

• 社団法人日本農芸化学会の論文
• 2003-08-23

著者

• Doi Hiroshi

  Department Of Medicine Juntendo University School Of Medicine

• Kawaguchi Masahumi

  Department Of Applied Biology Kyoto Institute Of Technology Matsugasaki

• Doi Hiroshi

  Department Of Cardiology Yokohama Rosai Hospital

• Doi H

  Department Of Food Science And Nutrition Mukogawa Women's University

• Timasheff S

  Graduate Department Of Biochemistry Brandeis University

• Kawaguchi Manami

  Department Of Food Science And Nutrition Mukogawa Women's University

• KAWAGUCHI Makiko

  Department of Food Science and Nutrition, Mukogawa Women's University

• TIMASHEFF Serge

  Graduate Department of Biochemistry, Brandeis University

• Kawaguchi M

  Department Of Food Science And Nutrition Mukogawa Women's University

• Kawaguchi Makiko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Doi Hiroshi

  Department Of Agricultural Chemistry Kyoto Prefectural University

• Kawaguchi Makiko

  Department Of Dermatology Faculty Of Medicine University Of Tokyo

• KAWAGUCHI MAKIKO

  Department of Anesthesiology, School of Dentistry, Aichi-Gakuin University

関連論文

• PE-040 Notch Signaling Enhances BMP2-induced Msx2 Gene Expression and Vascular Calcification(Atherosclerosis, basic-5, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-474 BMP2 and Notch Signaling Synergistically Induce the Expression of the Osteogenic Homeobox Transcription Factor Msx2 Gene(Atherosclerosis, basic-5 (H) PJ80,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circ
• Method for Purification of Fluorescence-Labeled Oligosaccharides by Pyridylamination(Biochemistry & Molecular Biology)
• PJ-118 Runx2 Represses TGF β/Smad-mediated Connective Tissue Growth Factor Gene Expression in Vascular Smooth Muscle Cells(PJ020,Atherosclerosis, Basic 1 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Societ
• Prostaglandin F2α Inhibits SERCA2 Gene Transcription Through an Induction of Egr-1 in Cultured Neonatal Rat Cardiac Myocytes
• Rationale and Design of a Study to Examine Lower Targets for Low-Density Lipoprotein-Cholesterol and Blood Pressure in Coronary Artery Disease Patients : The Japanese Coronary Artery Disease Study II (JCADII)
• PJ-118 Runx2 Represses TGF β/Smad-mediated Connective Tissue Growth Factor Gene Expression in Vascular Smooth Muscle Cells(PJ020,Atherosclerosis, Basic 1 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Societ
• Morning home blood pressure may be a significant marker of nephropathy in Japanese patients with type 2 diabetes : ADVANCED-J study 1
• Characterization of Wheat Germ Agglutinin Ligand on Soluble Glycoproteins in Caenorhabditis elegans
• Structural Analysis of N-Linked Glycans in Caenorhabditis elegans
• PJ-784 Identification of Cardiovascular Cell-specific DEPP as a Target Gene of Notch Signaling(Endothelium-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-716 Fibroblast Growth Factor 2 Induces Osteoblastic Phenotype in Vascular Smooth Muscle Cells(Vascular smooth muscle-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-710 Notch Signaling Accelerates Vascular Smooth Muscle Differentiation by Inducing Myocardin Gene Expression and by Enhancing the Transactivating Function of Myocardin(Vascular smooth muscle-2, The 71st Annual Scientific Meeting of the Japanese Circula
• OJ-196 Notch Signaling Promotes Differentiation of Circulating Vascular Progenitor Cells into Smooth Muscle Cells(Atherosclerosis, basic-3, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• OE-360 Fatty Acid Binding Protein 4 is a Target Gene of Notch Signaling in Endothelial Cells of Cardiac Capillaries(Endothelium-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• Catheter ablation of tachycardias after surgical atriotomy : Conventional method without electroanatomical mapping system
• Multi-site left atrial pacing with circumferential pulmonary vein catheter reveals multiple breakthrough points of pulmonary vein and differentiates far-field potential
• PE-345 Notch Signaling Induces Arterial Specific Maker Ephrin B2 in Venous Cell Line(Molecular Biology, Vascular 3 (H) : PE59)(Poster Session (English))
• OJ-197 HERP1 and HERP2, target genes of Notch, were induced during dedifferentiation of vascular smooth muscle cells(Atherosclerosis, Basic 2 (IHD) : OJ22)(Oral Presentation (Japanese))
• FRS-033 Expression of Erythropoietin Receptor is Induced in Human Failing Myocardium(Clinical Heart Failure, Diagnosis and Treatment-2 (M) FRS7,Featured Research Session,The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• Treatment of Spontaneous Intracranial Hypotension Secondary to C-2 Meningeal Cyst by Surgical Packing
• Trauma Sites and Clinical Features Associated With Acute Hyperextension Spinal Cord Injury Without Bone Damage : Relationship Between Trauma Site and Severity
• FRS-082 Cbfa1 Represses Vascular Smooth Muscle Cells Differentiation by Disrupting the SRF-myocardin Complex(Molecular Biology in Cardiovascular Diseases (basic), The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-099 COUP-TF2, an Orphan Nuclear Receptor, Inhibits Myocardin-induced Smooth Muscle Differentiation(Molecular biology, vascular-3 (H) PJ17,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• PE-421 Fatty Acid Binding Protein 4 is a Target Gene of Notch Signaling in Endothelial Cells(Molecular biology, vascular-2 (H) PE71,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
• OJ-249 Cbfa1, an Osteoblast-specific Transcription Factor, Represses SRF-mediated Smooth Muscle Cell Gene Transcription in Mesenchymal Stem Cells(Vascular smooth muscles-1 (H) OJ42,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meetin
• OJ-248 Jagged1-selective Activation of Notch Signaling Induces Vascular Smooth Muscle Cell Differentiation through RBP-Jk-dependent Mechanism(Vascular smooth muscles-1 (H) OJ42,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of
• OJ-215 Bone-Forming Transcription Factor Cbfa1 Induces Osteoblstic Differentiation and Respresses SMC Phenotype in Vascular SMC(Atherosclerosis, basic-2 (H) OJ36,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese C
• OE-303 DII4-selective Notch Signaling Induces EphrinB2 Gene Expression through HERP Independent Mechanisms in Endothelial Cells(Development and differentiation-1 (M) OE51,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Ja
• Notch Signaling is not Sufficient for Phenotypic Conversion from Vein to Artery (Endothelium/NO 3 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• Crosstalk between Notch and TGF-β Signaling Partially Contributes to Arterial Differentiation(Development and Differentiation 1 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• FGF-2 Induces Osteoblastic Marker Gene Expression through an Increase in DNA Binding Activity of cbfa-1 in Vascular Smooth Muscle Cells(Atherosclerosis, Basic 3 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• HERP1, a Target Gene of Notch, Maintains Undifferentiated State of Vascular Smooth Muscle Cells by Competing SRF Co-activator, Myocardin(Atherosclerosis, Basic 2 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
• IS195 Long-term Safety and Efficacy of Beta-blocker Therapy in Severer Valvular Aortic Stenosis
• Efficacy of Directional Coronary Atherectomy Prior to Stenting forthe Ostial Lesion of the Left Anterior Descending Coronary Artery
• Losartan Therapy in Patients with Chronic Severe Mitral Regurgitation
• Electrophysiological characteristics and effect of slow pathway ablation in atrioventricular nodal reentrant tachycardia with continuous atrioventricular conduction curves
• Limitation of Rota-stent for heavily calcified coronary lesions in diabetic patients
• Arrhythmogenic substrate in short-coupled variant of torsade de pointes
• Is Microtubule Assembly not Associated with GTP Hydrolysis?(Biological Chemistry)
• Polymerization and Calcium Binding of the Tubulin-colchicine Complex in the GDP State(Biochemistry & Molecular Biology)
• Ultracentrifugal behaviors of the tubulin-colchicine complex in the state of GTP in different buffers
• Enzymatic Synthesis of a Capsinoid by the Acylation of Vanillyl Alcohol with Fatty Acid Derivatives Catalyzed by Lipases(Food & Nutrition Science)
• Calcium binding to the tubulin-colchicine complex in the state of GTP in a BES buffer
• Comparative Analysis of Concentrations of trans- and cis-Resveratrol in Must and Wine from Eleven Different Grapevines Grown in Hokkaido, Northern Japan
• Cerebrosides in Grapevine Leaves : Distinct Composition of Sphingoid Bases Among the Grapevine Species Having Different Tolerances to Freezing Temperature
• Cyst of the Ligamentum Flavum : Case Report
• Isolation of an Antioxidant from PenicilIium roquefortii IFO 5956
• Occult Intrasacral Meningocele with Tethered Cord : Case Report
• Multisignature Schemes Using Structured Group ID
• Effect of calcium on the polymerization of the tubulin-colchicine complex in a BES buffer
• Brain Stem Abscess Treated Successfully by Ultrasound-guided Aspiration
• Disseminated cutaneous and visceral Kaposi's sarcoma in a patient with rheumatoid arthritis receiving corticosteroids and tacrolimus
• De novo 19q13.42 duplications involving NLRP gene cluster in a patient with systemic-onset juvenile idiopathic arthritis
• Breakpoint determination of X;autosome balanced translocations in four patients with premature ovarian failure
• A Novel SACS Mutation in an Atypical Case with Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay (ARSACS)
• Missense mutations in the DNA-binding/dimerization domain of NFIX cause Sotos-like features
• A family of oculofaciocardiodental syndrome (OFCD) with a novel BCOR mutation and genomic rearrangements involving NHS
• A girl with early-onset epileptic encephalopathy associated with microdeletion involving CDKL5
• Long-term Sequential Changes of Radiation Proctitis and Angiopathy in Rats
• Identification of a Novel Homozygous SPG7 Mutation in a Japanese Patient with Spastic Ataxia: Making an Efficient Diagnosis Using Exome Sequencing for Autosomal Recessive Cerebellar Ataxia and Spastic Paraplegia
• Participation of the Hydrophobic Bond in Complex Formation Between κ-Casein and β-Lactoglobulin
• Heat-induced Complex Formation between κ-Casein and α-Lactalbumin
• EXTRACTION OF RISK FACTORS OF POSTOPERATIVE VOMITING (POVs) FOLLOWING PALATOPLASTY
• Fulminant Pneumonia Caused by Nontypable Haemophilus Influenzae
• Effect of Carbohydrate Moiety of κ-Casein on the Complex Formation with β-Lactoglobulin
• Sibling cases of moyamoya disease having homozygous and heterozygous c.14576G>A variant in RNF213 showed varying clinical course and severity
• Utility of Thallium-201 Scintigraphy in Tolosa-Hunt Syndrome
• The diagnostic utility of exome sequencing in Joubert syndrome and related disorders

もっと見る 閉じる

スポンサーリンク