Effect of calcium on the polymerization of the tubulin-colchicine complex in a BES buffer

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概要

• 論文の詳細を見る

• The most fundamental property of tubulin is microtubule assembly, and then the polymerization reaction is the basic in the case of the tubulin-colchicine complex. Comparing the results carried out under the different conditions is important, because microtubule formation is affected by the condition. The polymerization was observed above at a concentration of 4 and 6 mM MgCl_2 at pH 6.5 and 7.0, respectively, in a BES buffer. The increase of magnesium concentration induced the high turbidity due to the polymerization. We indicated calcium ion inhibited the polymerization at low concentration. The presence of calcium ion induced the increase of critical concentration for polymerization. We also observed, however, some tendency of acceleration of the polymerization due to a high concentration of calcium ion even in the presence of any concentration of magnesium ion. The ratio of inhibition by calcium was much more dependent on the magnesium concentration, especially the change of the inhibitory ratio was big at pH 7.0.

• 武庫川女子大学の論文

著者

• Doi Hiroshi

  Department Of Cardiology Yokohama Rosai Hospital

• Kawaguchi Makiko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Doi H

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Doi Hiroshi

  Department Of Agricultural Chemistry Kyoto Prefectural University

• Kawaguchi Makiko

  Department Of Dermatology Faculty Of Medicine University Of Tokyo

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