Total DNA Preparation Excluding Extracellular Acidic Polysaccharide from Lipomyces Yeasts and its Application to Taxonomic Studies(Microbiology & Fermentation Technology)
スポンサーリンク
概要
- 論文の詳細を見る
A preparation method of total DNA from Lipomyces yeasts was improved in order to exclude extracellular acidic polysaccharide thoroughly. The method combined an ultracentrifuge and polyethylene glycol precipitation with the usual method. The total DNAs obtained were analyzed for G + C content and by DNA-DNA hybridization. The results all agreed almost completely with literature data. All the DNA samples prepared using this method were pure enough for these taxonomic analyses and could also be used as templates of PCR for amplification of small subunit ribosomal DNA and the internal transcribed spacer region.
- 社団法人日本農芸化学会の論文
- 2002-07-23
著者
-
SHIMADA KOJI
Department of Ocean Sciences, Faculty of Marine Science, Tokyo University of Marine Science and Tech
-
NISHIMURA Keiichiro
Research Institute for Advanced Science and Technology, Osaka Prefecture University
-
Nishimura K
Research Institute For Advanced Science And Technology Osaka Prefecture University
-
UZUKA YASUYUKI
Department of Fermentation Technology, Faculty of Engineering, Yamanashi University
-
Uzuka Y
The University Of The Air Yamanashi Study Center
-
Nishimura Katsushi
Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University
-
IWASAWA Hiroki
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
-
NAGANUMA Takafumi
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
-
Naganuma Takatoshi
Life Environment Medical Engineering Division Of Medical And Engineering Science Interdisciplinary G
-
Iwasawa Hiroki
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
-
Shimada Koji
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
関連論文
- Vertical Double Silicate Maxima in the Sea-Ice Reduction Region of the Western Arctic Ocean : Implications for an Enhanced Biological Pump due to Sea-Ice Reduction
- Insecticidal and Neural Activities of Candidate Photoaffinity Probes for Neonicotinoid Binding Sites(Organic Chemistry)
- Detection of ALMB-toxin in the Larval Body of Myrmeleon bore by Anti-N-terminus Peptide Antibodies
- Establishment of a Hepatocyte Cell Line Producing Growth-promoting Factors for Liver-colonizing Tumor Cells
- Suppression by Water Extracts of Sophora Plants of Sucrose-induced Hyperglycemia in Rats and Inhibition of Intestinal Disaccharidases In Vitro
- Structural Basis for Thermostability of Endo-1,5-α-L-Arabinanase form Bacillus thermodenitrificans TS-3
- Crystal Structure of Chloroplastic Ascorbate Peroxidase from Tobacco Plants and Structural Insights into its Instability
- Method for the Determination of Sulfite Resistance in Wine Yeasts
- Study on the Koji Mold Producing Substrate : Possible Application as a Food Supplement
- Fractionation and Anti-tumor Activity of the Mycelia of Liquid-cultured Phellinus linteus
- Analysis of mitochondrial DNA restriction fragment length polymorphisms for examining genetic variability among isolates of Phellinus linteus
- Structure-Depolarizing Activity Relationship for Achatin-I, a Tetrapeptide with a D-Phe Residue, and Its Derivatives toward the Crayfish Giant Axon
- Insecticidal activity of imidacloprid derivatives with an alkoxy group at the C5 position of the pyridine ring
- Preparation of Low Sodium Chloride Containing Soy Sauce Using Amino Acid Based Saltiness Enhancers
- Structure-Activity Relationships of RGD Mimetics as Fibrinogen-Receptor Antagonists
- Large Saphenous Vein Graft Aneurysm with a Fistula to the Right Atrium
- Production, Purification, and Properties of a Pectin Lyase from Pseudomonas marginalis N6301
- Heterogeneous material distribution, an important reason for generation of strain-localized mylonite and frictional slip zones in the Hidaka metamorphic belt, Hokkaido, Japan
- Occurrence of mylonite zones and pseudotachylyte veins around the base of the upper crust : An example from the southern Hidaka metamorphic belt, Samani area, Hokkaido, Japan
- Successful surgical treatment for infective endocarditis during pregnancy
- An Improved Method for Isolating Yeasts in the Genus Lipomyces and Related Genera from Soil
- Quantitative structure-activity relationships of imidacloprid and its analogs with substituents at the C5 position on the pyridine ring in the neuroblocking activity
- Asymmetric Chloronicotinyl Insecticide, 1-[1-(6-Chloro-3-pyridyl)ethyl]-2-nitroiminoimidazolidine : Preparation, Resolution and Biological Activities toward Insects and Their Nerve Preparations(Organic Chemistry)
- Prodrug-Oriented Molecular Design of Neonicotinoids : Preparation of Imidacloprid-Related 5,5-Dimethoxy-1,3-diazacyclohexane Derivatives and Their Insecticidal Activity
- Insecticidal and neuroblocking potencies of variants of the thiazolidine moiety of thiacloprid and quantitative relationship study for the key neonicotinoid pharmacophore
- Identification of Enzyme Responsible for Erythritol Utilization and Reaction Product in Yeast Lipomyces starkeyi(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Total DNA Preparation Excluding Extracellular Acidic Polysaccharide from Lipomyces Yeasts and its Application to Taxonomic Studies(Microbiology & Fermentation Technology)
- Growth of Wine Yeast and Formation of addition Compound of Sulfur Dioxide with Carbonyl Compounds
- Estimation of Heat Flux through the Eastern Bering Strait
- Quantitative Estimation of Intracellular Neutral Lipids of the Yeast, Lipomyces starkeyi
- Relation between Neutral Lipid Accumulation and the Growth Phase in the Yeast, Lipomyces starkeyi, a Fat Producing Yeast