Cloning and Nucleotide Sequence of the Mycodextranase Gene from Streptomyces sp. J-13-3(Biochemistry & Molecular Biology)

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Mycodextranase (EC 3. 2. 1. 61) is an α-glucanase that cleaves α-1, 4-bonds of alternating α-1, 3- and α-1, 4-linked D-glucan (nigeran). The gene encoding mycodextranase from Streptomyces sp. J-13-3 was cloned by hybridization with a degenerate oligonucleotide probe from the amino-terminal amino acid sequence of the enzyme and its nucleotide structure was analyzed. The open reading frame consisted of 1,803 base pairs encoding a signal peptide of 60 amino acids and a mature protein of 540 amino acids with a calculated molecular weight of 56,078. The deduced amino acid sequence showed weak similality to a chitinase homolog from Streptomyces lividans and a chitinase from Xanthomonas sp.
社団法人日本農芸化学会の論文
2001-07-23