Detection of Antitumor Promoting Activity in Raji Cells Carrying Epstein-Barr Virus Genome by Immunoblotting Analysis

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概要

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• Extract of Raji cells treated with sodium n-butyrate(1mM)and a tumor promoter, 12-0-tetradecanoylphorbol-13-acetate(TPA, 40ng/ml), was analyzed by immunoblotting using ten human sera with different antibody titers against Epstein-Barr virus early antigens. Two human sera reacted with one induced polypeptide of 48 kDa and its induction was inhibited by curcumin (4μg/ml), an antitumor promoter from turmeric. A mouse antiserum against P3HR-1 cells treated with TPA and sodium n-butyrate also detected the 48-kDa polypeptide in Raji cells treated with TPA at concentrations of 2.5 to 80ng/ml. These results indicate that the immunoblotting analysis can be used in a confirmation test for detection of antitumor promoting activity.

• 社団法人日本農芸化学会の論文
• 1998-02-23

著者

• KONDO Akira

  Department of Electrical and Electronic Engineering, Ehime University

• OKAZAKI Katsuichiro

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• Okazaki Katsuichiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• MORIMOTO Tetsuhiro

  Department of Life Sciences, Faculty of Agriculture, Kagawa University

• Okazaki Katsuichiro

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

• Morimoto Tetsuhiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Kondo Akira

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• Kondo Akira

  Department Of Agronomy Faculty Of Agriculture Tohoku University

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