Purification and Properties of Chitinase from Arthrobacter sp. NHB-10

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概要

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• A chitinase was purified from the culture filtrate of nigeran-degrading Arthrobacter sp. NHB-10 by precipitation with ammonium sulfate and column chromatographies on DEAE-Sephadex A-50 and Superose 12. The final preparation was homogenous in polyacrylamide gel electrophoresis. The molecular weight of the purified enzyme was 30,000 and its isoelectric point was 6.8. The optimum pH and temperature for the enzyme activity were 5.0 and 45℃, respectively. The enzyme was stable from pH 3 to 7 and up to 55℃. The enzyme activity was inhibited by Hg^<2+> and p-chloromercuribenzoic acid. Two internal amino acid sequences of the enzyme were AGPQLLTGYY and IGGVMT.

• 社団法人日本農芸化学会の論文
• 1999-09-23

著者

• Hayakawa Shigeru

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• OKAZAKI Katsuichiro

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• Hayakawa Shigeru

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

• Hayakawa Shigeru

  Department Of Biochemistry And Food Science Faculty Of Agriculture Kagawa University

• Hayakawa S

  School Of Food And Nutritional Sciences University Of Shizuoka

• Okazaki K

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Okazaki Katsuichiro

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

• KAWABATA Toshiyuki

  Department of Life Sciences, Faculty of Agriculture, Kagawa University

• NAKANO Masahito

  Department of Life Sciences Faculty of Agriculture, Kagawa University

• Nakano Masahito

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Okazaki Katsuichiro

  Department Of Applied Biological Science Faculty Of Agriculture Kagawa University

• Kawabata Tomohisa

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

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