Characteristics of an Aquenous Two-Phase System for α-Amylase Production
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概要
- 論文の詳細を見る
The characteristics of an aqueous two-phase system for the overproduction of extracellular enzyme through α-amylase fermentation by Bacillus amyloliquefaciens were investigated. With higher molecular weight of polyethylene glycol(PEG)or lower molecular weight of dextran, the partition coefficient of α-amylase was increased. α-Amylase biosynthesis was increased when PEG 6000 was included in the medium compared to the medium without PEG. Phosphate addition to the PEG-dextran system improved the partition coefficient of α-amylase, but deactivated α-amylase severely. By using sodium sulfate instead of phosphate, α-amylase deactivation was negligible, and high partitioning of the enzyme in the top phase was obtained.
- 公益社団法人日本生物工学会の論文
- 1991-05-25
著者
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Kim Sung
Department Of Anatomy
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Yoo Young
Department Of Anesthesiology And Pain Medicine Yonsei University Health System
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Yoo Young
Department Of Chemical Engineering Seoul National University
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Kim Sung
Department Of Chemical Engineering Seoul National University
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Yoo Young
Department of Advanced Materials Engineering, Yonsei University, Seoul 120-749, Republic of Korea
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Kim Sung
Department of Advanced Materials Engineering for Information and Electronics, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea
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