Purification and Characterization of Extracellular Medium-Chain-Length Polyhydroxyalkanoate Depolymerase from Pseudomonas sp.RY-1
スポンサーリンク
概要
- 論文の詳細を見る
A novel bacterial strain capable of growing in a medium containing a medium-chain-length polyhydroxyalkanoate (MCL-PHA) as the sole carbon source was isolated from a soil sample. The isolate, which was identified as Pseudomonas sp.RY-1, secreted MCL-PHA depolymerase into the culture fluid only when it was cultivated in a medium containing a MCL-PHA, such as polyhydroxyoctanoate (PHO) or polyhydroxynonanoate (PHN). The extracellular MCL-PHA depolymerase of this organism was purified to electrophoretic homogeneity. The enzyme was a tetramer with identical subunits and a total molecular mass of 115 kDa. The isoelectric point of this enzyme was estimated to be 5.9 by isoelectric focusing. The maximal activity was observed at pH 8.5 and 35℃. The enzyme was insensitive to phenylmethylsulfonyl fluoride and dithiothreitol, unlike other short-chain-length (SCL) PHA depolymerases. The K_m values for PHO and PHN were 0.86 and 1.47 mg/ml, respectively. The enzyme could not hydrolyze SCL-PHAs and p-nitrophenyl esters of fatty acids.
- 社団法人日本生物工学会の論文
- 2000-02-25
著者
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KIM Hyun
Department of Internal Medicine, Chonnam National University Hospital
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BAE Kyung-Sook
Korean Collection for Type Cultures, Korea Research Institute of Bioscience & Biotechnology
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Ryu Kang
Department Of Microbiology Chungnam National University
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Bae Kyung-sook
Korea Institute Of Bioscience And Biotechnology
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Rhee Young
Department Of Microbiology Chungnam National University
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Kim Hyun
Department Of Agricultural Biotechnology And Center For Agricultural Biomaterials Seoul National Uni
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Ryu Kang
Department Of Chemical Engineering Bioprocess Research Laboratory Hanyang University
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Kim Hyun
Department Of Agricultural Bio-resources National Academy Of Agricultural Science Rural Development
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Rhee Young
Department Of Microbiology And Molecular Biology Chungnam National University
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Kim Hyun
Department Of Microbiology Chungnam National University
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Kim Hyun
Department of Advanced Material Engineering, Daejeon University, Daejeon 300-716, Republic of Korea
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