Overexpression of the (R)-Specific Enoyl-CoA Hydratase Gene from Pseudomonas chlororaphis HS21 in Pseudomonas Strains for the Biosynthesis of Polyhydroxyalkanoates of Altered Monomer Composition
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概要
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The (<I>R</I>)-specific enoyl-CoA hydratase gene (<I>phaJ</I><SUB>HS21</SUB>) from <I>Pseudomonas chlororaphis</I> HS21 was overexpressed in various <I>Pseudomonas</I> strains, alone and in combination with the polyhydroxyalkanoate synthase gene (<I>phaC</I><SUB>HS21</SUB>), for the biosynthesis of polyhydroxyalkanoates (PHAs) of altered monomer composition. Recombinant <I>Pseudomonas</I> strains harboring <I>phaC</I><SUB>HS21</SUB> and <I>phaJ</I><SUB>HS21</SUB> generated saturated and unsaturated monomers of C12–C14 in their PHAs. In particular, the level of the 3-hydroxytetradecenoate monomer in recombinant <I>P. chlororaphis</I> HS21 increased by approximately 260%. PhaJ<SUB>HS21</SUB> is expected to be useful in the biosynthesis of PHAs consisting of unusual monomer units.
- Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
- 2012-03-23
著者
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Rhee Young
Department Of Microbiology And Molecular Biology Chungnam National University
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Chung Moon
Department Of Microbiology And Molecular Biology Chungnam National University
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- Overexpression of the (R)-Specific Enoyl-CoA Hydratase Gene from Pseudomonas chlororaphis HS21 in Pseudomonas Strains for the Biosynthesis of Polyhydroxyalkanoates of Altered Monomer Composition
- Overexpression of the (R)-Specific Enoyl-CoA Hydratase Gene from Pseudomonas chlororaphis HS21 in Pseudomonas Strains for the Biosynthesis of Polyhydroxyalkanoates of Altered Monomer Composition
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