Batch Production of High-Content Fructo-Oligosaccharides from Sucrose by the Mixed-Enzyme System of β-Fructofuranosidase and Glucose Oxidase

概要

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The production of high-content fructo-oligosaccharides from sucrose by the mixed-enzyme system of β-fructofuranosidase and glucose oxidase was investigated. The mixed-enzyme reaction was carried out in a stirred tank reactor containing 0.7 l of sucrose solution with coupled β-fructofuranosidase and glucose oxidase for 25h. The optimum conditions for the mixed-enzyme reaction were as follows : pH, 5.5; temperature, 40℃; sucrose concentration, 400 g/l; agitation speed, 551 rpm; oxygen flow rate, 0.7l/min; enzyme dosage, 10 units of β-fructofuranosidase with the combination of 15 units of glucose oxidase per gram sucrose. Under optimum conditions, high-content fructo-oligosaccharides up to 98% were obtained with complete consumption of sucrose and glucose by the mixed-enzyme system. Compared with the fructo-oligosaccharides produced by the β-fructofuranosidase, the high-content fructo-oligosaccharides produced by the mixed-enzyme system showed a significant difference with respect to suger composition; i.e., a higher content of nystose was accumulated and only a trace amount of fructofuranosyl nystose was detected.
社団法人日本生物工学会の論文
1994-02-25