A Comparative Study on Pb^<2+> Accumulation between Saccharomyces cerevisiae and Aureobasidium pullulans by SEM(Scanning Electron Microscopy) and EDX(Energy Dispersive X-Ray) Analyses

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Scanning electron microscopy and energy dispersive X-ray analyses were carried out in a comparative investigation of Pb^<2+> accumulation in Saccharomyces cerevisiae and Aureobasidium pullulans. In S.cerevisiae, the time required to reach an equilibrium state was shortened from 100 h to 1 h as the initial Pb^<2+> concentration decreased from 96.5 mg/ l to 16.0 mg/l, whereas the time was almost independent of initial Pb^<2+> concentration in A.pullulans. Concomitant with the Pb^<2+> accumulation, the cell surface of S.cerevisiae became rough and the amounts of potassium, phosphorus and sulfur on the cell surface decreased. However, significant increase of Pb^<2+> on the cell surface after Pb^<2+> accumulation was not observed due to Pb^<2+> penetration into the cell interior. In contrast, the Pb^<2+> accumulation had no significant effect on the surface characteristics of A.pullulans and extreme Pb^<2+> accumulation was observed on the cell surface because Pb^<2+> could not penetrate into the cell interior due to the existence of extracellular polymeric substances.
社団法人日本生物工学会の論文
1999-01-25