Non-Woven Fabrics as New Cell Matrices for IMR-90 Human Embryonic Lung Diploid Fibroblast Cells
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概要
- 論文の詳細を見る
IMR-90 cells could proliferate on non-woven fabrics (NWF) up to a cell density of about 3×10^5/cm^2,which was about 7 times higher compared to that in monolayer culture with a T-flask. The production of tissue plasminogen activator (t-PA) by perfusion culture using a device which includes a cultivating pan fitted inside a sheet of NWF was performed. The t-PA activity at a dilution rate of 0.22,0.33 and 0.45 d^<-1> was 245,220 and 160 IU/ml. Compared to plastic surfaces, NWF stably retained the cells during t-PA production using serum-free medium.
- 社団法人日本生物工学会の論文
- 1990-10-25
著者
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Suzuki Akira
Research Center for Compact Chemical System, National Institute of Advanced Industrial Science and T
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KAWANISHI Gosei
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
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Kawanishi G
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Kawanishi Gosei
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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MITSUDA SHINJIRO
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.,
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MATSUDA YOSHIAKI
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.,
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ITAGAKI YASUHARU
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.,
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KUMAZAWA EITARO
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.,
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HIGASHIO KANJI
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.,
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Higashio K
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Higashio Kanji
Reseach Institute Of Life Science Snow Brand Milk Products
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Mitsuda S
Snow Brand Milk Products Co. Ltd. Tochigi Jpn
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Mitsuda Shinjiro
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Itagaki Y
Sapporo Res. Lab. Sapporo‐shi Jpn
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Kumazawa E
Snow Brand Milk Products Co. Ltd. Tochigi Jpn
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Kumazawa Eitaro
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Matsuda Yoshiaki
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Suzuki A
The Femtosecond Technology Research Association
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Suzuki Akira
Research And Development Group Engineering Div.
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ITAGAKI YASUHARU
Research institute of Life Science, Snow Brand Milk Products Co., Ltd.
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