New Immobilization Method of Mammalian Cells Using Alginate and Polyacrylate
スポンサーリンク
概要
- 論文の詳細を見る
Mouse-mouse hybridoma cells were immobilized in polyacrylate-alginate gels. The immobilized hybridoma cells were cultured semi-continuously using a fluidized bed reactor, and allowed continuous antibody production without any gel destruction for one month. It has benn proved that the polyacrylate-alginate gels were tolerant against physical stress. The composition of the gels suitable for cell growth and antibody production was given as follows; viscosity of alginate at 1% solution : 60-100 cP, alginate concentration : 0.8%, and polyacrylate concentration : 0.2%. In the semi-continuous culture using gels prepared under suitable conditions, the viable cell number was estimated as 2.5×10^7 cells/ml-gel, and the antibody production rate was 2.2 mg/ml-gel/d, at maximum.
- 公益社団法人日本生物工学会の論文
- 1992-06-25
著者
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Mano Takashi
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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TAKESHITA Yasuyoshi
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd.
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Mitsuda Shinjiro
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Kumazawa Eitaro
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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Takeshita Yasuyoshi
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
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