Purification and Some Properties of β-Fructofuranosidase from Bifidobacterium adolescentis G1

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概要

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• A unique β-fructofuranosidase was purified from the extract of Bifidobacterium adolescentis G1 by anion-exchange, hydrophobic, and gel filtration chromatographies, and preparative electrophoresis. The molecular mass was 74kDa by SDS-PAGE, and the isoelectric point was pH 4.5. The enzyme was a monomeric protein. The pH optimum was at 6.1. The enzyme was stable at pH from 6.5 to 10.0, and up to 45℃. The neutral sugar content was 1.2%. The enzyme hydrolyzed 1-kestose faster than sucrose or inulin. The hydrolytic activity was strongly inhibited by Cu^<2+>, Ag^+ , Hg^+ , and p-chloromercuribenzoic acid. The K_m (mM) and k_o (s^<-1>) were : 1-kestose, 1.1 and 231 ; sucrose, 11 and 59.0 ; inulin, 8.0 and 149, respectively. From the kinetic results, β-fructofuranosidase from B. adolescentis G1 was concluded to have a high affinity for 1-kestose, thus differing from invertases and exo-inulinases in substrate specificity.

• 社団法人日本農芸化学会の論文
• 1993-10-23

著者

• KIKUCHI Masanori

  Department of Mechanical Engineering, Faculty of Science and Technology, Tokyo University of Science

• SHIOMI Norio

  Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido Universty

• ONODERA Shuichi

  Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University

• Kikuchi Masanori

  Department Of Food Production And Utility Development Graduate School Of Dairy Science Research Raku

• Shiomi Norio

  Department Of Food Production And Utility Development Graduate School Of Dairy Science Research Raku

• Shiomi Norio

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Kikuchi M

  Kikkoman Corp. Chiba Jpn

• Kikuchi Mamoru

  Res. And Dev. Div. Kikkoman Corp.

• Kikuchi M

  Res. And Dev. Div. Kikkoman Corp.

• Onodera S

  Dep. Of Food And Nutrition Sci. Graduate School Of Dairy Sci. Res. Rakuno Gakuen Univ.

• Onodera Shuichi

  Department Of Cardiology Shizuoka City Shizuoka Hospital

• Muramatsu Kei

  Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University

• Muramatsu K

  Kyoritsu Women's Univ. Tokyo Jpn

• Shiomi N

  Department Of Food And Nutrition Science Graduate School Of Dairy Science Research Rakuno Gakuen Uni

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