An Automatic Image Analyzing System for Evaluation of Elongating Behavior of Plant Hairy Roots Exposed to Herbicidal Stimuli (METHODS)

概要

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The elongating behavior of pak-bung hairy roots was evaluated by automatic tracing of the root tip point employing computer-aided image analysis. Though the root elongation rate in the absence of herbicides was approximately constant, the addition of 1.0μmol/dm^3 2,4-dichlorophenoxyacetic acid (2,4-D) or pyributicarb to the culture led to gradual deterioration of the elongation rate during measurements over a 25h period. The saturated value of the elongation rate (R^^<sat>__G) was determined as a measure to predict herbicidal toxicity to the roots by formulating the time profiles of the elongation rate. Under the conditions of 0.2-1.0μmol/dm^3 2,4-D and 0.1-10μmol/dm^3 pyributicarb, the dose-response profiles based on the R^^<sat>__G values overlapped closely with those based on the manually determined elongation rate in prolonged cultures for 168h. It was concluded that the system developed could be a useful tool for the assessment of herbicidal toxicity in the hairy roots.
社団法人日本生物工学会の論文
2003-07-25