TIME COURSE OF THE RECOVERY OF ADENOSINE TRIPHOSPHATE CONTENT WITH DENOSINE IN POST-ISCHEMIC HEARTS : A ^<31>P Magnetic Resonance Spectroscopy Study

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Restoration of the ATP level after brief ischemia is limited by slow de novo ATP synthesis and substrate loss in a salvage pathways for ATP synthesis, and complete recovery requires several days in the stunned heart. Adenosine is a substrate for myocardial ATP synthesis. We measured myocardial ATP in both ex vivo and in vivo experiments by ^<31>P magnetic resonance spectroscopy (^<31>P-MRS) and investigated the time course of ATP content recovery after application of adenosine. Guinea-pig perfused hearts were subjected to 30 min of global ischemia and reperfused for 3 h with 50 μM adenosine. The ATP level increased from 54±5% to 117±4% of the pre-ischemic value (<0.01). In dogs, we occluded the left anterior descending coronary artery for 40 min to produce regional ischemia. Afterwards, we administered 100 μmol/h adenosine into the left ventricle for 2 h. ATP levels increased from 63±4% to 77±5% of the pre-ischemic value with adenosine (p<0.05). However, ATP levels did not increase for a few hours after reperfusion both ex vivo and in vivo. Our ^<31>P-MRS studies demonstrated that brief ischemia depressed ATP levels and that administration of adenosine accelerated ATP formation in post-ischemic hearts.
社団法人日本循環器学会の論文
1994-07-20