THE DELETERIOUS EFFECTS OF EXOGENOUS ANGIOTENSION I AND ANGIOTENSIN II ON MYOCARDIAL ISCHEMIA-REPERFUSION INJURY

概要

論文の詳細を見る
Angiotensin II is well known to have a cardiotoxic effects. However, it is still unclear whether exogenous angiotensin I or angiotensin II has a deleterious effect on myocardial ischemia-reperfusion injury. To examine this deleterious effect, we administered angiotensin I and angiotensin II to perfused hearts before ischemia, and measured creatine kinase (CK) release and cardiac function during subsequent reperfusion. Wistar Kyoto rats were used and the hearts were perfused by the Langendorff technique at a constant flow (10 ml/min). Seven hearts were perfused for 20 min and then subjected to 15 min of global ischemia (Control). In the experimental groups, during the 5 min before ischemia, we administered 100 ng /ml angiotensin I (Ang I; n=9), 1 μg/ml enalaprilat (ACEI; n=5), both agents (ACEI+Ang I) (n=6) , or 10 ng/ml angiotensin II (Ang II; n = 6) . The perfusates were then sampled to measure angiotensin II. After 15 min of ischemia, the hearts were reperfused with control perfusate. Throughout the 20 min of reperfusion, the effluent was collected to measure cumulative CK release. Angiotensin I increased coronary perfusion pressure (CPP) by 32±4 mmHg, however, the angiotension converting enzyme inhibitor inhibited the increase of CPP by angiotension I (11±1 mmHg) (p<0.01). The contents of angiotensin II in the effluent in Ang I and Ang I+ACEI were 11.5±1.9 ng/ml and 4.0±0.5 ng/ml (p<0.01). After 20 min of reperfusion, the left ventricular developed pressure was unchanged in all of the groups. CPP was also unchanged by ischemia in all of the groups. Ventricular fibrillation was occurred only in Ang I and Ang II, with an incidence of 44% and 33% , respectively. The cumulative CK in Control, Ang I, ACEI, Ang I+ACEI, and Ang II was 7±1 IU, 19±2 IU,** 7±1 IU, 12±1 IU^+ and 20±2** IU. (**; p<0.01 vs Control.^+ ; p<0.05 vs Ang I). Angiotensin I (100 ng/ml) and angiotensin II (10 ng/ml) both produced ischemia-reperfusion injuries. Angiotensin I is converted to angiotensin II in cardiac tissue, and this angiotensin II is supposed to have a deleterious effect on ischemia-reperfusion injury. This harmful effect may not be related to the vasoconstriction induced by angiotensin II.
社団法人日本循環器学会の論文
1994-04-20

THE DELETERIOUS EFFECTS OF EXOGENOUS ANGIOTENSION I AND ANGIOTENSIN II ON MYOCARDIAL ISCHEMIA-REPERFUSION INJURY

スポンサーリンク

概要

著者

関連論文

スポンサーリンク