Effect of Sodium Chloride on Prostaglandin E_2 Synthesis in Rat Renal Papillary Collecting Tubule Cells in Culture : THE 14th CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION

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To investigate the relationship of renal prostaglandin E_2 (PGE_2) synthesis to medullary interstitial osmolality, rat renal papillary collecting tubule (RPCT) cells in culture were exposed to media with different osmolaties. PGE_2 released into the media was measured by radioimmunoassay. Increasing osmolality with equiosmolar sodium chloride and urea from 300 to 900 mOsm slightly increased PGE_2 synthesis, whereas further increase above 1200 mOsm markedly inhibited PGE_2 synthesis in RPCT cells. Hyperosmolar media also inhibited calsium ionphore-stimulated PGE_2 synthesis, but did not inhibit arachidonate-stimulated PGE_2 synthesis. In contrast, decreasing osmolality from 1800 to 300 mOsm induced a prominent but transient increase in PGE_2 synthesis. This increase was abolished by TMB-8, an intracellular calcium antagonist, and trifluoperazine, a calmodulin antagonist. Osmolar increments with sodium chloride or urea alone resulted in the similar inhibition of PGE_2 synthesis by sodium chloride but not by urea. These results indicate that extracellular sodium chloride inhibits PGE_2 synthesis in RPCT cells at the step of arachidonic acid release, possibly at phospholipases, in a calcium-dependent manner.
社団法人日本循環器学会の論文
1985-09-20